IE-3 (ATCC® CRL-2463)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma:  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma:
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody specifically binds to mouse ZP2 glycoprotein (amino acids 114-129).
The antibody cross-reacts with rat zonae but not with non-rodent species.
Derivation
Spleen cells were fused with Sp2/0 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse ZP2 glycoprotein
Comments
Animals were immunized with particulate murine zonae pellucidae.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
During development and maturation, mammalian oocytes are surrounded by the zona pellucida, which in the mouse is comprised of three sulfated glycoproteins, ZP1, ZP2 and ZP3.
The zona pellucida mediates sperm binding at fertilization, provides a post-fertilization block to polyspermy, and facilitates passage of pre-implantation embryos down the oviduct.
The antibody specifically binds to mouse ZP2 glycoprotein (amino acids 114-129).
The antibody cross-reacts with rat zonae but not with non-rodent species.
Following passive immunization, this antibody completely blocks both in vivo and in vitro fertilization of subsequently ovulated mouse eggs.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 10 mM HEPES, 0.15 mg/ml oxaloacetate, 0.05 mg/ml pyruvate, 0.0082 mg/ml bovine insulin and 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 5 X 10 exp4 and 5 X 10 exp5 viable cells/ml.
Attached cells may be subcultured by scraping.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG2a
Name of Depositor J Dean
Passage History
The zona pellucida mediates sperm binding at fertilization, provides a post-fertilization block to polyspermy, and facilitates passage of pre-implantation embryos down the oviduct.
References

East IJ, et al. Monoclonal antibodies to the murine zona pellucida protein with sperm receptor activity: effects on fertilization and early development. Dev. Biol. 109: 268-273, 1985. PubMed: 3996750

East IJ, Dean J. Monoclonal antibodies as probes of the distribution of ZP-2, the major sulfated glycoprotein of the murine zona pellucida. J. Cell Biol. 98: 795-800, 1984. PubMed: 6699085

Rankin TL, et al. Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding. Development 125: 2415-2424, 1998. PubMed: 9609824

Greenhouse S, et al. Antibodies to human ZP3 induce reversible contraception in transgenic mice with 'humanized' zonae pellucidae. Hum. Reprod. 14: 593-600, 1999. PubMed: 2118547

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

East IJ, et al. Monoclonal antibodies to the murine zona pellucida protein with sperm receptor activity: effects on fertilization and early development. Dev. Biol. 109: 268-273, 1985. PubMed: 3996750

East IJ, Dean J. Monoclonal antibodies as probes of the distribution of ZP-2, the major sulfated glycoprotein of the murine zona pellucida. J. Cell Biol. 98: 795-800, 1984. PubMed: 6699085

Rankin TL, et al. Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding. Development 125: 2415-2424, 1998. PubMed: 9609824

Greenhouse S, et al. Antibodies to human ZP3 induce reversible contraception in transgenic mice with 'humanized' zonae pellucidae. Hum. Reprod. 14: 593-600, 1999. PubMed: 2118547