IgG-IB7 (ATCC® CRL-2418)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody is specific for the alpha subunit of farnesyltransferase in cultured mammalian cells.
It is useful for studying the presence of the alpha subunit of farnesyltransferase in cell extraxcts.
This antibody may be used as a control for the diagnostic test for choroideremia (CHM), an X-linked form of retinal degeneration, by immunoblot analysis (see ATCC CRL-2419).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the alpha subunit of farnesyltransferase
Cellular Products
immunoglobulin; monoclonal antibody; against the alpha subunit of farnesyltransferase
Comments
Animals were immunized with a GST-farnesyltransferase fusion protein.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for the alpha subunit of farnesyltransferase in cultured mammalian cells.
It is useful for studying the presence of the alpha subunit of farnesyltransferase in cell extraxcts.
This antibody may be used as a control for the diagnostic test for choroideremia (CHM), an X-linked form of retinal degeneration, by immunoblot analysis (see ATCC CRL-2419).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cultures between 5 X 10 exp4 and 5 X 10 exp5 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1
Name of Depositor JL Goldstein, YK Ho
References

MacDonald IM, et al. A practical diagnostic test for choroideremia. Ophthalmology 105: 1637-1640, 1998. PubMed: 9754170

Andres DA, et al. Mutational analysis of alpha-subunit of protein farnesyltransferase. Evidence for a catalytic role. J. Biol. Chem. 268: 1383-1390, 1993. PubMed: 8419339

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

MacDonald IM, et al. A practical diagnostic test for choroideremia. Ophthalmology 105: 1637-1640, 1998. PubMed: 9754170

Andres DA, et al. Mutational analysis of alpha-subunit of protein farnesyltransferase. Evidence for a catalytic role. J. Biol. Chem. 268: 1383-1390, 1993. PubMed: 8419339