R2-9A5 [R2-9A5.2A2.1A10] (ATCC® CRL-2357)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Culture Properties suspension
Biosafety Level 1
Applications
The hybridoma cell line R2-9A5 [R2-9A5.2A2.1A10] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.
The antibody is a useful tool for detection of mouse IL-12p75 by ELISA and Western Blot analysis.
Derivation
The hybridoma cell line R2-9A5 [R2-9A5.2A2.1A10] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.
Clinical Data
R2-9A5 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with recombinant mouse IL-12 p75.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p75
Cellular Products
immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p75
Comments
The hybridoma cell line R2-9A5 [R2-9A5.2A2.1A10] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.
The antibody is a useful tool for detection of mouse IL-12p75 by ELISA and Western Blot analysis.
R2-9A5 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with recombinant mouse IL-12 p75.
Complete Growth Medium Iscove's Modified Dulbecco's Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol and 100 U/ml recombinant human IL-6, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium.
Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain at between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG2b
Name of Depositor DH Presky
Year of Origin 1993
References

Wilkinson VL, et al. Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA. J. Immunol. Methods 189: 15-24, 1996. PubMed: 8576576

Basic Documentation
References

Wilkinson VL, et al. Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA. J. Immunol. Methods 189: 15-24, 1996. PubMed: 8576576