R2-9A5 [R2-9A5.2A2.1A10] (ATCC® CRL-2357)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The hybridoma cell line R2-9A5 [R2-9A5.2A2.1A10] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.
The antibody is a useful tool for detection of mouse IL-12p75 by ELISA and Western Blot analysis.
Derivation
The hybridoma cell line R2-9A5 [R2-9A5.2A2.1A10] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.
Clinical Data
R2-9A5 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with recombinant mouse IL-12 p75.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p75
Cellular Products
immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p75
Comments
The hybridoma cell line R2-9A5 [R2-9A5.2A2.1A10] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.
The antibody is a useful tool for detection of mouse IL-12p75 by ELISA and Western Blot analysis.
R2-9A5 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with recombinant mouse IL-12 p75.
Complete Growth Medium Iscove's Modified Dulbecco's Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol and 100 U/ml recombinant human IL-6, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium.
Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain at between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG2b
Name of Depositor DH Presky
Year of Origin 1993
References

Wilkinson VL, et al. Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA. J. Immunol. Methods 189: 15-24, 1996. PubMed: 8576576

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Wilkinson VL, et al. Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA. J. Immunol. Methods 189: 15-24, 1996. PubMed: 8576576