LK-4 (ATCC® CRL-2345)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The hybridoma cell line LK-4 secretes a mouse monoclonal antibody (IgG1k) that recognizes human platelet glycoprotein GPIIIa.
The antibody can differentiate platelet PLA1/PLA1, PLA1/PLA2 and PLA2/PLA2 genotypes on soluble platelet extracts; it can recognize but not differentiate intact platelets.
The antibody is useful in immunoblot and ELISA assays.
The line was produced by fusing P3U1 mouse myeloma cells with spleen cells from a BALB/c female mouse differentially immunized with human PLA2 platelets and cyclophosphamide, followed by PLA1 platelets.
Derivation
The line was produced by fusing P3U1 mouse myeloma cells with spleen cells from a BALB/c female mouse differentially immunized with human PLA2 platelets and cyclophosphamide, followed by PLA1 platelets.
Clinical Data
The line was produced by fusing P3U1 mouse myeloma cells with spleen cells from a BALB/c female mouse differentially immunized with human PLA2 platelets and cyclophosphamide, followed by PLA1 platelets.
Genes Expressed
immunoglobulin; monoclonal antibody; against human platelet glycoprotein GPIIIa.
Cellular Products
immunoglobulin; monoclonal antibody; against human platelet glycoprotein GPIIIa.
Comments
The hybridoma cell line LK-4 secretes a mouse monoclonal antibody (IgG1k) that recognizes human platelet glycoprotein GPIIIa.
The antibody can differentiate platelet PLA1/PLA1, PLA1/PLA2 and PLA2/PLA2 genotypes on soluble platelet extracts; it can recognize but not differentiate intact platelets.
The antibody inhibits binding of human anti- PLA1 antibody to human platelets.
The antibody is useful in immunoblot and ELISA assays.
The line was produced by fusing P3U1 mouse myeloma cells with spleen cells from a BALB/c female mouse differentially immunized with human PLA2 platelets and cyclophosphamide, followed by PLA1 platelets.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1; kappa light chain
Name of Depositor S Karpatkin
References

Liu LX, et al. A monoclonal antibody (LK-4) which differentiates PLA1 from PLA2 platelet extracts but not intact platelets. Thromb. Res. 66: 309-320, 1992. PubMed: 1384161

Liu LX, et al. Development of a monoclonal antibody capable of differentiating platelet PLA1/PLA1, PLA1/PLA2 and PLA2/PLA2 genotypes. Br. J. Haematol. 81: 113-117, 1992. PubMed: 1520609

Liu LX, et al. Heterogenous inhibition of platelet aggregation by monoclonal antibodies binding to multiple sites on GPIIIa. Br. J. Haematol. 91: 976-982, 1995. PubMed: 8547151

Valetin N, et al. Involvement of the cysteine-rich domain of glycoprotein IIIa in the expression of the human platelet alloantigen, PlA1: evidence for heterogeneity in the humoral response. Blood 85: 3028-3033, 1995. PubMed: 7756638

Liu LX, et al. Inhibition of binding of anti-PLA1 antibodies to platelets with monoclonal antibody LK-4. Evidence for multiple PLA1 receptor sites on platelet GPIIIa. Blood 88: 3601-3607, 1996. PubMed: 8896429

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Liu LX, et al. A monoclonal antibody (LK-4) which differentiates PLA1 from PLA2 platelet extracts but not intact platelets. Thromb. Res. 66: 309-320, 1992. PubMed: 1384161

Liu LX, et al. Development of a monoclonal antibody capable of differentiating platelet PLA1/PLA1, PLA1/PLA2 and PLA2/PLA2 genotypes. Br. J. Haematol. 81: 113-117, 1992. PubMed: 1520609

Liu LX, et al. Heterogenous inhibition of platelet aggregation by monoclonal antibodies binding to multiple sites on GPIIIa. Br. J. Haematol. 91: 976-982, 1995. PubMed: 8547151

Valetin N, et al. Involvement of the cysteine-rich domain of glycoprotein IIIa in the expression of the human platelet alloantigen, PlA1: evidence for heterogeneity in the humoral response. Blood 85: 3028-3033, 1995. PubMed: 7756638

Liu LX, et al. Inhibition of binding of anti-PLA1 antibodies to platelets with monoclonal antibody LK-4. Evidence for multiple PLA1 receptor sites on platelet GPIIIa. Blood 88: 3601-3607, 1996. PubMed: 8896429