MAb104 (ATCC® CRL-2067)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Storage Conditions liquid nitrogen vapor phase
Derivation

Animals were immunized with purified nuclei from Xenopus laevis oocytes.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed
Immunoglobulin; monoclonal antibody; against SR proteins (pre-mRNA splicing factors)
Cellular Products
Immunoglobulin; monoclonal antibody; against SR proteins (pre-mRNA splicing factors)
Comments

The antibody reacts with a conserved phosphorylated epitope on SR proteins.

SR proteins are a family of proteins that have pre-mRNA splicing activity (SR comes from the sequences of consecutive serine and arginine residues in these proteins). The family consists of at least 6 proteins with masses of 75000, 70000, 55000, 40000, 30000 and 20000 daltons.

Complete Growth Medium Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgM
Name of Depositor MB Roth
References

Zahler AM, et al. SR proteins: a conserved family of pre-mRNA splicing factors. Genes Dev. 6: 837-847, 1992. PubMed: 1577277

Roth MB, et al. A conserved family of nuclear phosphoproteins localized to sites of polymerase II transcription. J. Cell Biol. 115: 587-596, 1991. PubMed: 1717489

Zahler AM, et al. Distinct functions of SR proteins in alternative pre-mRNA splicing. Science 260: 219-222, 1993. PubMed: 8385799

Roth MB, et al. A monoclonal antibody that recognizes a phosphorylated epitope stains lampbrush chromosome loops and small granules in the amphibian germinal vesicle. J. Cell Biol. 111: 2217-2223, 1990. PubMed: 1703534

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Zahler AM, et al. SR proteins: a conserved family of pre-mRNA splicing factors. Genes Dev. 6: 837-847, 1992. PubMed: 1577277

Roth MB, et al. A conserved family of nuclear phosphoproteins localized to sites of polymerase II transcription. J. Cell Biol. 115: 587-596, 1991. PubMed: 1717489

Zahler AM, et al. Distinct functions of SR proteins in alternative pre-mRNA splicing. Science 260: 219-222, 1993. PubMed: 8385799

Roth MB, et al. A monoclonal antibody that recognizes a phosphorylated epitope stains lampbrush chromosome loops and small granules in the amphibian germinal vesicle. J. Cell Biol. 111: 2217-2223, 1990. PubMed: 1703534

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.