N3.36 (ATCC® CRL-2042)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with neonatal and adult fast class human and rodent skeletal muscle myosin.
Derivation
Animals were immunized with partially purified human neonatal (5 days postpartum) skeletal muscle myosin heavy chain.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against neonatal and adult fast myosin heavy chain (MyHC)
Cellular Products
immunoglobulin; monoclonal antibody; against neonatal and adult fast myosin heavy chain (MyHC)
Comments
Animals were immunized with partially purified human neonatal (5 days postpartum) skeletal muscle myosin heavy chain.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The antibody reacts with neonatal and adult fast class human and rodent skeletal muscle myosin.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM nonessential amino acids and 1 mM sodium pyruvate, 90%; iron supplemented bovine calf serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor HM Blau
References

Hughes SM, Blau HM. Muscle fiber pattern is independent of cell lineage in postnatal rodent development. Cell 68: 659-671, 1992. PubMed: 1531450

Cho M, et al. Evidence for myoblast-extrinsic regulation of slow myosin heavy chain expression during muscle fiber formation in embryonic development. J. Cell Biol. 121: 795-810, 1993. PubMed: 8491773

Basic Documentation
References

Hughes SM, Blau HM. Muscle fiber pattern is independent of cell lineage in postnatal rodent development. Cell 68: 659-671, 1992. PubMed: 1531450

Cho M, et al. Evidence for myoblast-extrinsic regulation of slow myosin heavy chain expression during muscle fiber formation in embryonic development. J. Cell Biol. 121: 795-810, 1993. PubMed: 8491773