A4.74 [4A.74] (ATCC® CRL-2041)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with the human fast MyHC and with rodent fast IIa MyHC.
It cross-reacts weakly with IIx MyHC and does not react with fast IIb MyHC isoform of rodent skeletal muscle myosin.
Derivation
Animals were immunized with partially purified human adult skeletal muscle myosin heavy chain.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human fast and rodent fast IIa myosin heavy chain (MyHC)
Comments
Animals were immunized with partially purified human adult skeletal muscle myosin heavy chain.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The antibody reacts with the human fast MyHC and with rodent fast IIa MyHC.
It cross-reacts weakly with IIx MyHC and does not react with fast IIb MyHC isoform of rodent skeletal muscle myosin.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM nonessential amino acids and 1 mM sodium pyruvate, 90%; iron supplemented bovine calf serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG1
Name of Depositor HM Blau
References

Hughes SM, Blau HM. Muscle fiber pattern is independent of cell lineage in postnatal rodent development. Cell 68: 659-671, 1992. PubMed: 1531450

Hughes SM, et al. Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle. Dev. Biol. 158: 183-199, 1993. PubMed: 7687223

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hughes SM, Blau HM. Muscle fiber pattern is independent of cell lineage in postnatal rodent development. Cell 68: 659-671, 1992. PubMed: 1531450

Hughes SM, et al. Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle. Dev. Biol. 158: 183-199, 1993. PubMed: 7687223