PS 38 (ATCC® CRL-1950)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody cross-reacts with the NP-OT of both mouse in both radioimmunoassays and immunohistochemical assays.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against rat oxytocin associated neurophysin (NP-OT)
Cellular Products
immunoglobulin; monoclonal antibody; against rat oxytocin associated neurophysin (NP-OT)
Comments
Animals were immunized with an acid soluble extract of rat (Sprague-Dawley) intermediate lobes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody cross-reacts with the NP-OT of both mouse in both radioimmunoassays and immunohistochemical assays.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, non-essential amino acids, 2 mM L-glutamine and 1 mM sodium pyruvate, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2b; kappa light chain
Name of Depositor H Gainer
References

Ben-Barak Y, et al. Neurophysin in the hypothalamo-neurohypophysial system. I. Production and characterization of monoclonal antibodies. J. Neurosci. 5: 81-97, 1985. PubMed: 3880813

Ben-Barak Y, et al. Phylogenetic cross-reactivities of monoclonal antibodies produced against rat neurophysin. Cell. Mol. Neurobiol. 4: 339-349, 1984. PubMed: 6085284

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Ben-Barak Y, et al. Neurophysin in the hypothalamo-neurohypophysial system. I. Production and characterization of monoclonal antibodies. J. Neurosci. 5: 81-97, 1985. PubMed: 3880813

Ben-Barak Y, et al. Phylogenetic cross-reactivities of monoclonal antibodies produced against rat neurophysin. Cell. Mol. Neurobiol. 4: 339-349, 1984. PubMed: 6085284