C44 (ATCC® CRL-1943)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody binds with high affinity to colchicine, and with lower affinity to related compounds.
It is useful for monitoring therapeutic drug levels and has the potential to reverse colchicine toxicity.
Derivation
The C44 hybridoma was originated by S.K. Edmond Rouan in 1987.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against colchicine
Cellular Products
immunoglobulin; monoclonal antibody; against colchicine
Comments
The C44 hybridoma was originated by S.K. Edmond Rouan in 1987.
Animals were immunized with a colchicine - keyhole limpet hemocyanin (KLH) conjugate.
Spleen cells were fused with NS-1 myeloma cells.
The antibody binds with high affinity to colchicine, and with lower affinity to related compounds.
The antibody is bound strongly by Protein A.
It is useful for monitoring therapeutic drug levels and has the potential to reverse colchicine toxicity.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2a
Name of Depositor AC Cunningham
References

Edmond Rouan SK, et al. Specific, high affinity colchicine binding monoclonal antibodies: development and characterization of the antibodies. Hybridoma 8: 435-448, 1989. PubMed: 2777275

Rouan SK, et al. Reversal of colchicine-induced mitotic arrest in Chinese hamster cells with a colchicine-specific monoclonal antibody. Am. J. Pathol. 137: 779-787, 1990. PubMed: 2221012

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Edmond Rouan SK, et al. Specific, high affinity colchicine binding monoclonal antibodies: development and characterization of the antibodies. Hybridoma 8: 435-448, 1989. PubMed: 2777275

Rouan SK, et al. Reversal of colchicine-induced mitotic arrest in Chinese hamster cells with a colchicine-specific monoclonal antibody. Am. J. Pathol. 137: 779-787, 1990. PubMed: 2221012