514 (ATCC® CRL-1914)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Leukemia

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Leukemia
Applications
Animals were immunized with BB2800 leukemia cells which produce both Friend MuLV (FrMuLV) and mink cell focus inducing (MCF) FrMuLV.
The antibody neutralizes most MCF and polytropic MuLV, and reacts well with live or fixed infected cells and with Western blots.
The antibody efficiently immunoprecipitates gp70, and can be used to distinguish polytropic MuLV from ecotropic, xenotropic and amphotropic MuLV.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against gp70 envelop antigen (ENV) protein of murine leukemia viruses (MuLV)
Comments
Animals were immunized with BB2800 leukemia cells which produce both Friend MuLV (FrMuLV) and mink cell focus inducing (MCF) FrMuLV.
Spleen cells were fused with NS-1 myeloma cells.
The antibody neutralizes most MCF and polytropic MuLV, and reacts well with live or fixed infected cells and with Western blots.
The antibody efficiently immunoprecipitates gp70, and can be used to distinguish polytropic MuLV from ecotropic, xenotropic and amphotropic MuLV.
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor B Chesebro
References

Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011