11E10 (ATCC® CRL-1907)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Animals were immunized with a formalinized toxoid prepared from crude SLT-II produced by an E. coli K-12 strain lysogenized with the 933W toxin converting phage of enterohemorrhagic E. coli 933.
The antibody reacts with the A subunit of SLT-II and does not react with B subunit.
The antibody can be used in SLT-II neutralization assays and in assays to detect SLT-II producing E. coli strains.
It may also be used to differentiate SLT-II from SLT-I, SLT-IIv and Shiga toxin.
The 11E10 antibody differs from that of 11F11 (ATCC CRL-1908) in that it can partially neutralize SLT-IIv.
Derivation
Animals were immunized with a formalinized toxoid prepared from crude SLT-II produced by an E. coli K-12 strain lysogenized with the 933W toxin converting phage of enterohemorrhagic E. coli 933.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Shiga like toxin II (SLT-II)
Cellular Products
immunoglobulin; monoclonal antibody; against Shiga like toxin II (SLT-II)
Comments
Animals were immunized with a formalinized toxoid prepared from crude SLT-II produced by an E. coli K-12 strain lysogenized with the 933W toxin converting phage of enterohemorrhagic E. coli 933.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the A subunit of SLT-II and does not react with B subunit.
The antibody can be used in SLT-II neutralization assays and in assays to detect SLT-II producing E. coli strains.
It may also be used to differentiate SLT-II from SLT-I, SLT-IIv and Shiga toxin.
The 11E10 antibody differs from that of 11F11 (ATCC CRL-1908) in that it can partially neutralize SLT-IIv.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype IgG1; kappa light chain
Name of Depositor AD O'Brien
References

Perera LP, et al. Isolation and characterization of monoclonal antibodies to Shiga-like toxin II of enterohemorrhagic Escherichia coli and use of the monoclonal antibodies in a colony enzyme-linked immunosorbent assay. J. Clin. Microbiol. 26: 2127-2131, 1988. PubMed: 3053764

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Perera LP, et al. Isolation and characterization of monoclonal antibodies to Shiga-like toxin II of enterohemorrhagic Escherichia coli and use of the monoclonal antibodies in a colony enzyme-linked immunosorbent assay. J. Clin. Microbiol. 26: 2127-2131, 1988. PubMed: 3053764