372 (ATCC® CRL-1893)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Leukemia

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Leukemia
Applications
Spleen cells from a mouse inoculated with a B-tropic Friend murine leukemia virus (FrMuLV) were fused with NS-1 myeloma cells.
The antibody is highly specific for p15E protein from most MuLV.
Derivation
Spleen cells from a mouse inoculated with a B-tropic Friend murine leukemia virus (FrMuLV) were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against p15E envelop antigen (ENV) protein of murine leukemia viruses (MuLV)
Cellular Products
immunoglobulin; monoclonal antibody; against p15E envelop antigen (ENV) protein of murine leukemia viruses (MuLV)
Comments
Spleen cells from a mouse inoculated with a B-tropic Friend murine leukemia virus (FrMuLV) were fused with NS-1 myeloma cells.
The antibody is highly specific for p15E protein from most MuLV.
The antibody efficiently immunoprecipitates p15E and works well in Western blotting assays for p15E.
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG3
Name of Depositor B Chesebro
References

Chesebro B, et al. Characterization of mouse monoclonal antibodies specific for Friend murine leukemia virus-induced erythroleukemia cells: Friend-specific and FMR-specific antigens. Virology 112: 131-144, 1981. PubMed: 6787798

Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011

Basic Documentation
References

Chesebro B, et al. Characterization of mouse monoclonal antibodies specific for Friend murine leukemia virus-induced erythroleukemia cells: Friend-specific and FMR-specific antigens. Virology 112: 131-144, 1981. PubMed: 6787798

Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011