3G9F3 (ATCC® CRL-1843)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody is specific for the gIII protein of PRV.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against pseudorabies virus (PRV)
Cellular Products
immunoglobulin; monoclonal antibody; against pseudorabies virus (PRV)
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with pseudorabies virus (IA strain 62-26).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for the gIII protein of PRV.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2b
Name of Depositor CA Whetstone