HP6014 (ATCC® CRL-1752)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The HP6014 antibody represents the only known IgG2 subclass specific reagent for the Fab region.
This antibody was selected as a reference reagent of the WHO Collaborating Center at the Centers for Disease Control for Human Immunoglobulin Subclasses.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified human immunoglobulin.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the Fd portion of human IgG2 (Fab region)
Cellular Products
immunoglobulin; monoclonal antibody; against the Fd portion of human IgG2 (Fab region)
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with purified human immunoglobulin.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The HP6014 antibody represents the only known IgG2 subclass specific reagent for the Fab region.
This antibody was selected as a reference reagent of the WHO Collaborating Center at the Centers for Disease Control for Human Immunoglobulin Subclasses.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 10 mM HEPES and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor CB Reimer
References

Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201

Jefferis R, et al. Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol. Lett. 10: 223-252, 1985. PubMed: 3899923

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

The depositor has provided these cells for research purposes only and with the following restrictions: (1) no distribution should be made to third parties, (2) any proposed commercial use must be negotiated with the Centers for Disease Control, (3) all papers reporting any use of these or derived clones should make direct reference to the original publication (Hybridoma 3:263-275, 1984).

References

Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201

Jefferis R, et al. Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol. Lett. 10: 223-252, 1985. PubMed: 3899923