BR1H-826.5 [PTA-4302] (ATCC® CRL-13020)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte; somatic cell hybri  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
These antibodies can be used to detect BRCA1 mutations.
Animals were immunized with purified bacterially expressed fusion proteins containing BRCA1 amino acids 1360 to 1555. Spleen cells were fused with the mouse myeloma cell line P3.653. Monoclonal antibodies BR1H-788.6 (ATCC PTA-4301), BR1H-945.2 (ATCC PTA-4303) and BR1H-826.5 (ATCC PTA-4302) are against a portion of a BRCA1 polypeptide between the N-terminal and C-terminal regions of the BRCA1 polypeptide (amino acids 1360 to 1555).
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with purified bacterially expressed fusion proteins containing BRCA1 amino acids 1360 to 1555. Spleen cells were fused with the mouse myeloma cell line P3.653. Monoclonal antibodies BR1H-788.6 (ATCC PTA-4301), BR1H-945.2 (ATCC PTA-4303) and BR1H-826.5 (ATCC PTA-4302) are against a portion of a BRCA1 polypeptide between the N-terminal and C-terminal regions of the BRCA1 polypeptide (amino acids 1360 to 1555). Monoclonal antibodies BR1N.129.5 (ATCC PTA-4304) and BR1N-411.4 (ATCC PTA-4305) are against the N-terminal region of a BRCA1 polypeptide (amino acids 1 to 304). Monoclonal antibodies BR1S-218.1 (ATCC PTA-4307), BR1S-060.2 (ATCC PTA-4306), BR1S-384.5 (ATCC PTA-4308), and BR1S-425.1 (ATCC PTA-4309) are against the C-terminal region of a full-length BRCA1 polypeptide (amino acids 1840-1862). These antibodies can be used to detect BRCA1 mutations.
Comments
Animals were immunized with purified bacterially expressed fusion proteins containing BRCA1 amino acids 1360 to 1555. Spleen cells were fused with the mouse myeloma cell line P3.653. Monoclonal antibodies BR1H-788.6 (ATCC PTA-4301), BR1H-945.2 (ATCC PTA-4303) and BR1H-826.5 (ATCC PTA-4302) are against a portion of a BRCA1 polypeptide between the N-terminal and C-terminal regions of the BRCA1 polypeptide (amino acids 1360 to 1555). Monoclonal antibodies BR1N.129.5 (ATCC PTA-4304) and BR1N-411.4 (ATCC PTA-4305) are against the N-terminal region of a BRCA1 polypeptide (amino acids 1 to 304). Monoclonal antibodies BR1S-218.1 (ATCC PTA-4307), BR1S-060.2 (ATCC PTA-4306), BR1S-384.5 (ATCC PTA-4308), and BR1S-425.1 (ATCC PTA-4309) are against the C-terminal region of a full-length BRCA1 polypeptide (amino acids 1840-1862). These antibodies can be used to detect BRCA1 mutations.
Complete Growth Medium HH4 Medium supplemented with 10% fetal bovine serum
Cryopreservation
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype mouse IgG1
Name of Depositor Hybritech, Inc.
References

Knott CL, Kuus-Reichel K. Methods of detecting BRCA1 mutations. U.S. Patent 6,514,713 dated Feb 4 2003

Basic Documentation
References

Knott CL, Kuus-Reichel K. Methods of detecting BRCA1 mutations. U.S. Patent 6,514,713 dated Feb 4 2003