Crypthecodinium cohnii (Seligo) Javornicky (ATCC® 50299)

Organism: Crypthecodinium cohnii (Seligo) Javornicky  /  Depositor: CA Beam, M Himes

Permits and Restrictions

View Permits

Strain Designations GP1
Application
Biofuel production
Biosafety Level 1
Isolation
Zostera sp., Greenport, Long Island, NY
Product Format frozen
Type Strain no
Comments
Reproductively isolated from the WH group. Indistinguishable from strains of major sibling species WH based upon zymogram patterns of soluble enzymes and SSU rRNA sequences.
Minor Sibling Species Gp
Medium ATCC® Medium 460: A2E6 medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 30021 SPEC: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCC medium 460 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen one half turn, and incubate culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 460 and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss.
Subcultivation
Protocol: ATCCNO: 30021 SPEC: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCC medium 460 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen one half turn, and incubate culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 460 and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss.
Name of Depositor CA Beam, M Himes
References

Beam CA, Himes M. Electrophoretic characterization of members of the Crypthecodinium cohnii (Dinophyceae) species complex. J. Protozool. 34: 204-217, 1987.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation