Crypthecodinium cohnii (Seligo) Javornicky (ATCC® 30342)

Organism: Crypthecodinium cohnii (Seligo) Javornicky  /  Depositor: CA Beam, M Himes

Permits and Restrictions

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Strain Designations M 7
Application
Biofuel production
Biosafety Level 1
Isolation
rotting Fucus sp., Mattapoisett, MA, 1973
Product Format frozen
Type Strain no
Comments
Major Sibling Species WH
genetic diversification
cryopreservation
use of plastic ampoules for freeze preservation
Medium ATCC® Medium 460: A2E6 medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 30021 SPEC: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCC medium 460 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen one half turn, and incubate culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 460 and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss.
Subcultivation
Protocol: ATCCNO: 30021 SPEC: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCC medium 460 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen one half turn, and incubate culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 460 and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss.
Name of Depositor CA Beam, M Himes
Year of Origin 1973
References

Salt GW. Changes in the cell volume of Didinium nasutum during population increase. J. Protozool. 22: 112-115, 1975.

Beam CA, Himes M. Sexual isolation and genetic diversification among some strains of Crypthecodinium cohnii-like dinoflagellates evidence of speciation. J. Protozool. 24: 532-539, 1977.

Beam CA, Himes M. Evidence for sexual fusion and recombination in the dinoflagellate Crypthecodinium (Gyrodinium) cohnii. Nature 250: 435-436, 1974. PubMed: 4851938

Simione FP Jr., Daggett PM. Recovery of a marine dinoflagellate following controlled and uncontrolled freezing. Cryobiology 14: 362-366, 1977. PubMed: 560941

Simione FP Jr., et al. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502, 1977. PubMed: 891238

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation