pCFH (ATCC® PVMC-6)

Organism: Beet curly top virus  /  Clone Type: Clone  /  Depositors: DC Stenger

Permits and Restrictions

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Designations pCFH
Species Beet curly top virus
Depositors DC Stenger
Applications
Contains sequence
Plant research
Vector
Construct size (kb): 5.99
Construction of the clone:  Plasmid constructed by inserting 2927 bp EcoRI linearized viral dsDNA into EcoRI site of pUC8vector.
Insert
DNA: cDNA
Insert lengths(kb): 2.927
Tissue: beet severe curly top virus
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Plasmid constructed by inserting 2927 bp EcoRI linearized viral dsDNA into EcoRI site of pUC8 vector. The clone is infectious. ATCC sample contains 50 µg of purified plasmid DNA in 50 µL TE buffer.
Classification Curtovirus
References

Stenger DC. Complete nucleotide sequence of the hypervirulent CFH strain of beet curly top virus. Mol. Plant-Microbe Interact. 7: 154-157, 1994. PubMed: 8167369

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