Murine hepatitis virus (ATCC® VR-765)

Classification: Nidovirales, Coronaviridae, Coronavirus,Group 2  /  Product Format: frozen

Classification Nidovirales, Coronaviridae, Coronavirus,Group 2
Deposited As Mouse hepatitis virus
Agent Murine hepatitis virus
Strain MHV-JHM
Common Name Murine hepatitis virus
Biosafety Level 2
Isolation
tissue, animal
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Effect on Host
Yes, in vitro effects: cytopathic effects in tissue culture, fusing and clumping of cells with lots of floating cells
CPE, fusing and clumping cells, cells floating
Recommended Host

Recommended Host: NCTC clone 1469 cells (ATCC® CCL-9.1™)   

Growth Conditions
Atmosphere: 5% CO2 in air recommended
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 70% - 80% confluent (not 100% confluent).
Temperature: 37°C
Cryopreservation
Storage temperature: -70°C
Effect on Host
Yes, in vitro effects: cytopathic effects in tissue culture, fusing and clumping of cells with lots of floating cells
CPE, fusing and clumping cells, cells floating
Name of Depositor J Parker
Special Collection ATCC
Source
tissue, animal
References

Cheever FS, et al. A murine virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin. J. Exp. Med. 90: 181-194, 1949.

or colder

brain of 17-day-old Swiss mouse with spontaneous flaccid paralysis of hind legs

or colder