Murine hepatitis virus (ATCC® VR-765™) Classification: Nidovirales, Coronaviridae, Coronavirus,Group 2 / Product Format: frozen General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Classification Nidovirales, Coronaviridae, Coronavirus,Group 2 Agent Murine hepatitis virus Strain MHV-JHM Common Name Murine hepatitis virus Biosafety Level 2 Isolation tissue, animal Product Format frozen Storage Conditions -70°C or colder (or colder) Effect on Host Yes, in vitro effects: cytopathic effects in tissue culture, fusing and clumping of cells with lots of floating cellsCPE, fusing and clumping cells, cells floating Recommended Host Recommended Host: NCTC clone 1469 cells (ATCC® CCL-9.1) Growth Conditions Atmosphere: 5% CO2 in air recommended Recommendations for Infection: For best results cells should be 24 to 48 hours old and 70% - 80% confluent (not 100% confluent). Temperature: 37°C Cryopreservation Storage temperature: -70°C Effect on Host Yes, in vitro effects: cytopathic effects in tissue culture, fusing and clumping of cells with lots of floating cellsCPE, fusing and clumping cells, cells floating Name of Depositor J Parker Special Collection ATCC Source tissue, animal References Cheever FS, et al. A murine virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin. J. Exp. Med. 90: 181-194, 1949. or colder brain of 17-day-old Swiss mouse with spontaneous flaccid paralysis of hind legs or colder Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS