Betacoronavirus 1 (ATCC® VR-1558)

Classification: Coronaviridae, Betacoronavirus  /  Product Format: frozen 1 mL per vial

Classification Coronaviridae, Betacoronavirus
Deposited As Human coronavirus OC43
Agent Betacoronavirus 1
Strain OC43
Common Name Human coronavirus OC43
Applications
Respiratory research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Derived by adaptation from in vivo product ATCC® VR-759™ to HCT8 [HRT18] cells (ATCC® CCL-244™). ATCC® VR-759™ and ATCC® VR-1558™ have not been compared for sequence or infectivity in in vivo and tissue culture. No antigenic cross reaction with Human coronavirus 229E (ATCC® VR-740™).
Effect on Host
CPE, vacuolization of cytoplasm and sloughing
Recommended Host
HCT-8 [HRT-18] (ATCC® CCL-244™)
Growth Conditions Temperature: 33°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere while rocking continuously. End adsorption by adding virus growth medium.
Incubation: 4-6 days
Effect on Host
CPE, vacuolization of cytoplasm and sloughing
Name of Depositor JO Fleming, University of Wisconsin, Madison; ATCC®
Special Collection ATCC
Chain of Custody ATCC <-- JO Fleming <-- MK Cooney <-- AZ Kapikian
Source Man with cold-like illness. Treated to remove mycoplasma at ATCC.
References

Bruckova M, et al. The adaptation of two human coronavirus strains (OC38 and OC43) to growth in cell monolayers. Proc. Soc. Exp. Biol. Med. 135: 431-435, 1970. PubMed: 4991398

McIntosh K, et al. Growth in suckling-mouse brain of "IBV-like" viruses from patients with upper respiratory tract disease. Proc. Natl. Acad. Sci. USA 58: 2268-2273, 1967. PubMed: 4298953

McIntosh K, et al. Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease. Proc. Natl. Acad. Sci. USA 57: 933-940, 1967. PubMed: 5231356