Influenza A virus (H1N1) (ATCC® VR-1737™) Classification: Orthomyxoviridae, Influenzavirus A / Product Format: frozen 1 mL per vial General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Classification Orthomyxoviridae, Influenzavirus A Agent Influenza A virus (H1N1) Strain A/Virginia/ATCC2/2009 Applications Emerging infectious disease researchRespiratory researchInfluenza Research Biosafety Level 2 Product Format frozen 1 mL per vial Storage Conditions -70°C or colder Images Comments Item analyzed using Real-time RT-PCR to determine strain is of pandemic swine Influenza A origin. Effect on Host CPE, rounded refractile cells with cell degeneration Recommended Host MDCK (ATCC® CCL-34™) Growth Conditions Temperature: 35°C Recommendations For Infection: Plate cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted to provide a MOI 0.1. Adsorb for 1 hour at 35°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 2-5 days Effect on Host CPE, rounded refractile cells with cell degeneration Name of Depositor ATCC Special Collection ATCC Source Nasopharyngeal specimen from a clinical patient, 2009 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS Other Documentation Micrograph of Host Effects