Influenza A virus (H3N2) (ATCC® VR-1680™)

Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen 1 mL per vial

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Classification Orthomyxoviridae, Influenzavirus A
Agent Influenza A virus (H3N2)
Strain A/Aichi/2/68 (TC-adapted)
Applications
Emerging infectious disease research
Respiratory research
Influenza Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments
ATCC® VR-547™ and ATCC® VR-1680™ have not been compared with respect to sequence or infectivity in chick embryo and tissue culture.
Effect on Host
CPE, cell rounding, degeneration, sloughing
Recommended Host
MDCK (ATCC® CCL-34™)
Growth Conditions
Temperature: 35°C
Recommendations For Infection: For best results, plate cells 24-48 hours in advance. Infect at 80-90% confluent (not 100% confluent) using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 35°C in a humidified, 5% CO2 atmosphere for 2-4 days, until CPE are well advanced in 100% of the culture. In TC, ATCC® VR-1680 should be grown in serum free media that contains 1 ug/mL TPCK-treated trypsin, 0.125% BSA and 1% HEPES (1 molar stock).
Incubation: 2-4 days
Effect on Host
CPE, cell rounding, degeneration, sloughing
Name of Depositor MT Coleman and ATCC
Source Isolated from sailor on Israeli ship docking at Aichi, Japan, 1968
References

Coleman MT, et al. The Hong Kong-68 influenza A2 variant. Lancet 2: 1384-1386, 1968. PubMed: 4177941

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS