Influenza A virus (H1N1) (ATCC® VR-1672)

Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen

Classification Orthomyxoviridae, Influenzavirus A
Agent Influenza A virus (H1N1)
Strain Monoclonal Anti-Influenza Virus H1 Hemagglutinin (HA), A/South Carolina/1/1918 (H1N1), clone 58C4 (produced in vitro)
Applications
Emerging infectious disease research
Respiratory research
Influenza Research
Biosafety Level 1
Product Format frozen
Storage Conditions  -20°C or colder Repeated freeze-thaw cycles should be avoided
Comments
Antibody Class: IgG2bκ
The B cell hybridoma was generated by the fusion of Sp2/0 BALB/c mouse myeloma cells with splenocytes from mice immunized by DNA vaccination with a plasmid encoding the HA of influenza virus, A/South Carolina/1/1918 (H1N1) and then boosted with whole inactivated virus.
Name of Depositor A Garcia-Sastre, Mount Sinai School of Medicine, New York, New York
Source
Mouse monoclonal antibody prepared against the H1 hemagglutinin (HA) of influenza virus, A/South Carolina/1/1918 (H1N1) was purified from clone 58C4 hybridoma supernatant by protein G affinity chromatography.
References

Glaser L, et al. A single amino acid substitution in 1918 influenza virus hemagglutinin changes receptor binding specificity. J. Virol. 79: 11533-11536, 2005. PubMed: 16103207

Manicassamy B, et al. Protection of mice against lethal challenge with 2009 H1N1 influenza A virus by 1918-like and classical swine H1N1 based vaccines. PLoS Pathog. 6: e1000745, 2010. PubMed: 20126449

Tumpey TM, et al. Existing antivirals are effective against influenza viruses with genes from the 1918 pandemic virus. Proc. Natl. Acad. Sci. USA. 99: 13849-13854, 2002. PubMed: 12368467