Human respiratory syncytial virus (ATCC® VR-1540)

Classification: Paramyxoviridae, Pneumovirus  /  Product Format: frozen 1 mL per vial

Classification Paramyxoviridae, Pneumovirus
Deposited As Respiratory syncytial virus
Agent Human respiratory syncytial virus
Strain A2
Applications
Respiratory research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images Human respiratory syncytial virus ATCC VR-1540 images
Comments VR-1540 was prepared from ATCC VR-1302; by growth in the presence of neutralizing serum to remove contaminating Human adenovirus 1.
Effect on Host
CPE, syncytial formation, rounding, and sloughing
Recommended Host
HEp-2 (ATCC CCL-23)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, syncytial formation, rounding, and sloughing
Name of Depositor L Potash, Flow Laboratories, Inc.; ATCC
Special Collection NCRR Contract
Source Lower respiratory tract of infant with bronchiolitis and bronchopneumonia, Melbourne, Australia, 1961
References

Cameron R, et al. Identification of contaminating adenovirus type 1 in the ATCC reference strain of respiratory syncytial virus A2 (VR-1302). Virus Res. 92: 151-156, 2003. PubMed: 12686423

Lewis FA, et al. A syncytial virus associated with epidemic disease of the lower respiratory tract in infants and young children. Med. J. Aust. 2: 932-933, 1961.