Hs 895.Sk (ATCC® CRL-7636™)

Organism: Homo sapiens, human  /  Cell Type: fibroblast,Melanoma

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Cell Type fibroblast,Melanoma
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 48 years
Gender female
Ethnicity Caucasian
Applications
Thus, the two lines provide tumor and normal counterparts for comparative in vitro studies.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 46; range = 32 to 46
Derivation
This normal skin fibroblast cell line was isolated from the same patient as Hs 895.T (ATCC CRL-7637), a melanoma. Thus, the two lines provide tumor and normal counterparts for comparative in vitro studies.
Clinical Data
This normal skin fibroblast cell line was isolated from the same patient as Hs 895.T (ATCC CRL-7637), a melanoma.
female
Caucasian
48 years
Comments
Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
This normal skin fibroblast cell line was isolated from the same patient as Hs 895.T (ATCC CRL-7637), a melanoma. Thus, the two lines provide tumor and normal counterparts for comparative in vitro studies.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 90%; fetal bovine serum, 10%
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: 1:2 to 1:4
Medium Renewal: Every 2 to three days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

The line was established from apparently normal tissue from a patient who had metastatic melanoma.