HCC1395 (ATCC® CRL-2324™)

Organism: Homo sapiens, human  /  Tissue: mammary gland; breast/duct  /  Cell Type: Epithelial, lymphoblast

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Tissue
mammary gland; breast/duct
Cell Type Epithelial, lymphoblast
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease TNM stage I, grade 3, primary ductal carcinoma
Age 43 years adult
Gender female
Ethnicity Caucasian, White
Applications
HCC1395 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.
The cells are negative for expression of estrogen receptors (ER -) according to depositor and negative for expression of progesterone receptors (PR -).
The cells are negative for expression of Her2-neu but positive for expression of p53.
The cells are poorly differentiated and vacuolated.
Storage Conditions liquid nitrogen vapor phase
Karyotype polyploid
Derivation
This cell line was initiated on 12/14/94 from a patient with a family history of cancer (patient's mother had breast cancer).
Clinical Data
43 years adult
An EBV transformed lymphoblastoid cell line (HCC1395BL) from the same patient is available as ATCC CRL-2325.
Caucasian, White
female
This cell line was initiated on 12/14/94 from a patient with a family history of cancer (patient's mother had breast cancer).
Receptor Expression
estrogen receptor, negative according to depositor
progesterone receptor, negative
Oncogene her2/neu -, p53 +
Genes Expressed
Epithelial glycoprotein 2 (EGP2),cytokeratin 19,her2/neu -, p53 +,The cells are negative for expression of Her2-neu but positive for expression of p53.,The cells are negative for expression of estrogen receptors (ER -) according to depositor and negative for expression of progesterone receptors (PR -).
Comments
The cell line took 14 months to establish.
The tumor was classified as TNM stage I, grade 3, invasive ductal carcinoma with 0 out of 34 lymph node metastasis.
The cells are poorly differentiated and vacuolated.
The cells are negative for expression of Her2-neu but positive for expression of p53.
HCC1395 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.
The cells are negative for expression of estrogen receptors (ER -) according to depositor and negative for expression of progesterone receptors (PR -).
An EBV transformed lymphoblastoid cell line (HCC1395BL) from the same patient is available as ATCC CRL-2325.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 9
D16S539: 11
D5S818: 11
D7S820: 10,12
THO1: 9.3
TPOX: 11
vWA: 15
Name of Depositor AF Gazdar, AK Virmani
Year of Origin December 14, 1994
References

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Cancer cell line mutation data
Restrictions The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.
References

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771