HCC1143 (ATCC® CRL-2321™)

Organism: Homo sapiens, human  /  Tissue: mammary gland; breast; duct  /  Cell Type: Epithelial

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Tissue
mammary gland; breast; duct
Cell Type Epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease TNM stage IIA, grade 3, primary ductal carcinoma
Age 52 years adult
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype polyploid
Derivation
HCC1143 is a poorly differentiated cell line that was initiated on August 30, 1994; it took 29 months to establish.
HCC1143 was derived from an invasive ductal carcinoma with no lymph node metastases.
Clinical Data
52 years adult
Caucasian
female
Oncogene her2/neu -; p53 +
Genes Expressed
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19,her2/neu -; p53 +,The cells are negative for expression of Her2-neu but positive for expression of p53.,HCC1143 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR).
Comments
HCC1143 was derived from an invasive ductal carcinoma with no lymph node metastases.
The cells are negative for expression of Her2-neu but positive for expression of p53.
HCC1143 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, rinse with 0.25% trypsin, 0.53 mM EDTA solution, add an additional 1 to 2 mL of trypsin solution and allow the flask to set at room temperature (or incubate at 37°C) until cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 3 to 4 days
Cryopreservation
Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 11,13
D5S818: 11
D7S820: 12
THO1: 9.3
TPOX: 12
vWA: 16
Name of Depositor AF Gazdar, AK Virmani
Year of Origin August 30, 1994
References

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Cancer cell line mutation data
Restrictions The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.
References

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771