COLO 829BL (ATCC® CRL-1980)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed  /  Tissue: peripheral blood  /  Disease: normal

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Organism Homo sapiens, human
Tissue peripheral blood
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2  [Cells contain Herpesvirus]
Disease normal
Age 45 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation

The specimen was taken from a patient prior to chemotherapy for metastatic melanoma.

The COLO 829BL cell line was established at the ATCC by Epstein-Barr virus (EBV) transformation of of a sample of peripheral blood provided by G.E. Moore.

A cell line was also produced from the melanoma, and is available as COLO 829 (ATCC CRL-1974).
Clinical Data
45 years
male
Caucasian
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 4 x 105 viable cells/mL. Maintain cell density between 2 x 105 and 2 x 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Name of Depositor GE Moore
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation