KEL FIB (ATCC® CRL-1762™)

Organism: Homo sapiens, human  /  Tissue: skin/connective tissue  / 

Organism Homo sapiens, human
Tissue
skin/connective tissue
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease keloid
Age 35 years
Gender female
Ethnicity Black
Clinical Data
35 years
Black
female
Genes Expressed
collagen
Comments
Progeny from the current distribution stock (passage 11) have been shown to undergo at least 10 population doublings from the frozen state.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse monolayer with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X
CSF1PO: 8,12
D13S317: 11,13
D16S539: 9
D5S818: 12
D7S820: 8,10
THO1: 7,8
TPOX: 8,11
vWA: 14,16
Name of Depositor M Babu, RF Diegelmann, I Cohen
Passage History
Progeny from the current distribution stock (passage 11) have been shown to undergo at least 10 population doublings from the frozen state.