WM-115 (ATCC® CRL-1675)

Organism: Homo sapiens, human  /  Tissue: skin  /  Disease: melanoma

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Organism Homo sapiens, human
Tissue skin
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease melanoma
Age 58 years adult
Gender female
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a hypotetraploid human cell line. The modal chromosome number is 87, occurring at 50%, with higher ploidies at 1.6%. Eight markers [der(1)t(1;9) (p31.2;q11), 6p+, del(6) (p21.1), t(12p14q) and four others] were found in all cells. Of these markers, six (including all four listed above) were consistently paired and two others were single. Normal N6 and N9 were absent and N7 had six copies per cell. There were only two X chromosomes per cell.
Another cell line derived from a metastasis in the same patient also is available (ATCC CRL-1676).
The WM-115 cell line was derived from the primary tumor.
Clinical Data
58 years
Genes Expressed
proteoglycan antigen characteristic of melanoma
Cellular Products
proteoglycan antigen characteristic of melanoma
Tumorigenic Yes
Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells.
Comments Another cell line derived from a metastasis in the same patient also is available (ATCC CRL-1676).
The cells should be incubated at 34°C to 35°C.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.03% EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 34 to 35°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 34 to 35°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Freeze Medium: Complete growth medium 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 34°C; (Max. 35°C, Min. 34°C)
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 12,13
D16S539: 11,12
D5S818: 13
D7S820: 8
THO1: 7,9
TPOX: 8,11
vWA: 15,17
Name of Depositor M Herlyn
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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