5C6 Clone 1 (ATCC® CRL-1969)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with the mouse myelomonocytic adhesion promoting complement receptor (CR3, CD11b).
Derivation
Spleen cells were fused with Y3 myeloma cells.
Clinical Data
It inhibits myelomonocytic adhesion in vitro, and recruitment of inflammatory cells in vivo.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse CD11b (type 3 complement receptor, CR3)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse CD11b (type 3 complement receptor, CR3)
Comments
Animals were immunized with thioglycollate stimulated mouse peritoneal macrophages.
Spleen cells were fused with Y3 myeloma cells.
The antibody reacts with the mouse myelomonocytic adhesion promoting complement receptor (CR3, CD11b).
It inhibits myelomonocytic adhesion in vitro, and recruitment of inflammatory cells in vivo.
Complete Growth Medium Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
50% FBS + 40% complete growth medium + 10% DMSO
Isotype IgG2b
Name of Depositor H Rosen, S Gordon
References

Rosen H. Role of CR3 in induced myelomonocytic recruitment: insights from in vivo monoclonal antibody studies in the mouse. J. Leukocyte Biol. 48: 465-469, 1990. PubMed: 2230596

Hutchings P, et al. Transfer of diabetes in mice prevented by blockade of adhesion- promoting receptor on macrophages. Nature 348: 639-642, 1990. PubMed: 2250718

Rosen H, Gordon S. Monoclonal antibody to the murine type 3 complement receptor inhibits adhesion of myelomonocytic cells in vitro and inflammatory cell recruitment in vivo. J. Exp. Med. 166: 1685-1701, 1987. PubMed: 2445894

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rosen H. Role of CR3 in induced myelomonocytic recruitment: insights from in vivo monoclonal antibody studies in the mouse. J. Leukocyte Biol. 48: 465-469, 1990. PubMed: 2230596

Hutchings P, et al. Transfer of diabetes in mice prevented by blockade of adhesion- promoting receptor on macrophages. Nature 348: 639-642, 1990. PubMed: 2250718

Rosen H, Gordon S. Monoclonal antibody to the murine type 3 complement receptor inhibits adhesion of myelomonocytic cells in vitro and inflammatory cell recruitment in vivo. J. Exp. Med. 166: 1685-1701, 1987. PubMed: 2445894