Dengue virus type 2 (ATCC® VR-1584)

Classification: Flaviviridae, Flavivirus, Dengue virus  /  Product Format: frozen 1 mL per vial

Classification Flaviviridae, Flavivirus, Dengue virus
Agent Dengue virus type 2
Strain New Guinea C
Applications
Vector borne research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Derived by adaptation from in vivo product ATCC® VR-1255™ to LLC-MK2 Derivative cells (ATCC® CCL-7.1™). ATCC® VR-1255™ and ATCC® VR-1584™ have not been compared for sequence or infectivity in in vivo and tissue culture.
Effect on Host CPE, cell aggregation, sloughing, death
Recommended Host LLC-MK2 Derivative (ATCC® CCL-7.1™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 6-8 days
Effect on Host CPE, cell aggregation, sloughing, death
Name of Depositor CL Wisseman, University of Maryland School of Medicine; Yale Arbovirus Research Unit; National Institute of Allergy and Infectious Diseases; ATCC®
Source Serum of febrile patient, New Guinea, 1944
References

Sabin AB. Research on dengue during World War II. Am. J. Trop. Med. Hyg. 1: 30-50, 1952. PubMed: 14903434