Plasmodium falciparum Welch (ATCC® 50110)

Strain Designations: HB-2  /  Depositor: W Trager  /  Biosafety Level: 2

Strain Designations HB-2
Emerging infectious disease research
Vector borne research
Biosafety Level 2
derived from existing strain
Isolation date: 1981
Product Format frozen
Type Strain no
This is a non-gametocyte-forming strain.
Medium ATCC® Medium 2196: Malaria medium, complete
Growth Conditions
Temperature: 37.0°C
Growth condition: in vitro culture in human erythrocytes. Consult product sheet for protocol.


        Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust.

1.     Centrifuge ring stage culture for 5 min at 1800rpm in 50 ml centrifuge tube.

2.     Aspirate supernatant using sterile Pasteur pipet.

3.     Resuspend pellet gently in remaining supernatant.

4.     Slowly add 5 volumes of glycerolyte medium (see below) to 3 volumes pellet dropwise via a syringe as follows:

        A.    Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.

B.    Add the remaining 4 volumes of glycerolyte and gently agitate.

5.     Aliquot mixture into Nunc screwtop freezing vials and store at –80oC overnight.

6.     Plunge vials into liquid nitrogen (-196oC) the next day and store in liquid nitrogen or liquid nitrogen vapor.

** To formulate glycerolyte medium, combine the following with distilled water to 100 ml:  57.00g glycerol, 1.60g sodium lactate (C3H5NaO3), 30.00mg potassium chloride (KCl), 1.38g sodium dihydrogen phosphate (NaH2PO4). Mix well and adjust pH to 6.8 using concentrated NaOH and/or HCl. Autoclave to sterilize, and store at 4oC.

Name of Depositor W Trager
Special Collection ATCC
Year of Origin 1981

Bhasin VK, Trager W. Gametocyte-forming and non-gametocyte-forming clones of Plasmodium falciparum. Am. J. Trop. Med. Hyg. 33: 534-537, 1984. PubMed: 6383092