Plasmodium falciparum Welch (ATCC® 30950)

Strain Designations: Honduras-1/CDC  /  Depositor: W Trager  /  Biosafety Level: 2

Permits and Restrictions

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Strain Designations Honduras-1/CDC
Application
Emerging infectious disease research
Vector borne research
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
human, Cholutec, Honduras, 1980
Product Format frozen
Type Strain no
Antibiotic Resistance
pyrimethamine-resistant, chloroquine-sensitive
Comments
pyrimethamine-resistant, chloroquine-sensitive
Medium ATCC® Medium 2196: Malaria medium, complete
Growth Conditions
Temperature: 37.0°C
Growth condition: in vitro culture in human erythrocytes. Consult product sheet for protocol.
Cryopreservation

CRYOPRESERVATION: 

Only young cells (rings) can be frozen in glycerolyte medium* because their membranes are more robust.

1.     Centrifuge ring-stage culture for 5 mins. at 1000 x g in 50 ml centrifuge tube.

2.     Aspirate supernatant using sterile Pasteur pipet.

3.     Resuspend pellet gently in remaining supernatant.

4.     Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows:

        A.    Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.

B.    Add the remaining 4 volumes of glycerolyte and gently agitate.

5.   Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.).

6.   Plunge vials into liquid nitrogen (-196oC) the next day and store in liquid nitrogen or liquid nitrogen vapor.

       

Name of Depositor W Trager
Chain of Custody
ATCC <<--W Trager<<--CDC
Year of Origin 1980
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation