Plasmodium falciparum Welch (ATCC® 30932)

Organism: Plasmodium falciparum Welch  /  Depositor: W Trager

Strain Designations FCR-3/FMG [ FCR-3/Gambia)]
Application
Emerging infectious disease research
Vector borne research
Biosafety Level 2
Isolation Human clinical specimen, Fajara Gambia, 1976
Product Format frozen
Storage Conditions Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain no
Medium Medium 2196: Malaria medium, complete
Growth Conditions
Temperature: 37°C
Culture System: In vitro culture in human erythrocytes
Cryopreservation Reagents
Glycerolyte medium**
Glycerol: 57.00 g
NaC3H5O3 (Sodium lactate): 1.60 g
KCl: 30.00 mg
NaH2PO4: 1.38 g
Glass distilled water: 100.00 mL
Mix well and adjust pH to 6.8 using concentrated NaOH and/or HCl. Autoclave and store at 4°C.

Harvesting and Preservation
Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust.
  1. Centrifuge ring-stage culture for 5 mins at 1000 x g in 50 mL centrifuge tube.
  2. Aspirate supernatant using sterile Pasteur pipet.
  3. Resuspend pellet gently in remaining supernatant.
  4. Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows:
    1. Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
    2. Add the remaining 4 volumes of glycerolyte and gently agitate.
  5. Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1°C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.).
  6. Plunge vials into liquid nitrogen (-196°C) the next day and store in liquid nitrogen or liquid nitrogen vapor.
Name of Depositor W Trager
Special Collection ATCC
Year of Origin 1976
References

Isolated from an infected human blood sample collected by M. Friedman, Medical Research Council Labs Clinic, Fajara, Gambia, 1976.

Jensen JB, et al. Clinical drug-resistant falciparum malaria acquired from cultured parasites. Am. J. Trop. Med. Hyg. 30: 523-525, 1981. PubMed: 7020443

Jensen JB, Trager W. Plasmodium falciparum in culture: establishment of additional strains. Am. J. Trop. Med. Hyg. 27: 743-746, 1978. PubMed: 356635

Jensen JB, Trager W. Plasmodium falciparum in culture: use of outdated erthrocytes and description of the candle jar method. J. Parasitol. 63: 883-886, 1977. PubMed: 335035

Basic Documentation
FAQs
  1. ATCC item no. 30932 Type O blood supplier
    ATCC obtains O type blood to grow the Plasmodium falciparum from Interstate Blood Bank.  For more information, please contact Tech Services at
    Date Updated: 12/6/2013