Trypanosoma sp. (ATCC® 30510)

Strain Designations: Indonesian 71 triple clone  /  Depositor: D Weinman  /  Biosafety Level: 2

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Strain Designations Indonesian 71 triple clone
Application
Vector borne research
Biosafety Level 2
Isolation
Macaca irus, Indonesia, 1967
Product Format frozen
Type Strain no
Medium Medium 449: Diphasic blood agar base medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor D Weinman
Year of Origin 1967
References

Weinman D, Wiratmadja NS. The first isolates of trypanosomes in Indonesia and in history from primates other than man. Trans. R. Soc. Trop. Med. Hyg. 63: 497-506, 1969. PubMed: 5821271

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