Babesia microti (Franca) Reichenow (ATCC® 30222)

Depositor: JP Kreier  /  Biosafety Level: 2

Permits and Restrictions

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Application
Vector borne research
Biosafety Level 2
Isolation
forest mouse, Thamnomys surdaster, Congo Kinshasa, Zaire, 1950
Product Format frozen
Type Strain no
Comments
ATCCNO: 30221 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject the entire contents intraperitoneally into a young hamster. Remove parasitized blood via cardiac puncture and inject 2.5 X 10(6) or greater parasitized erythrocytes from donor into the appropriate host at weekly intervals.
Growth Conditions
Duration: in vivo, laboratory mouse
Protocol: ATCCNO: 30221 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject the entire contents intraperitoneally into a young hamster. Remove parasitized blood via cardiac puncture and inject 2.5 X 10(6) or greater parasitized erythrocytes from donor into the appropriate host at weekly intervals.
Subcultivation
Protocol: ATCCNO: 30221 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject the entire contents intraperitoneally into a young hamster. Remove parasitized blood via cardiac puncture and inject 2.5 X 10(6) or greater parasitized erythrocytes from donor into the appropriate host at weekly intervals.
Cryopreservation

Alsever's Solution

NaCl                                                                                    4.2 g

Na3citrate·2H2O                                                                8.0 g

Glucose                                                                            20.5 g

Glass distilled H2O to                                                        1.0 L

Name of Depositor JP Kreier
Chain of Custody
ATCC <<--JP Kreier<<--W. Ketschmar <<--- M. Schindler <<--- . . . <<--- I. Vincke (?)
Year of Origin 1950
References

Ann. Soc. Belge Med. Trop. 30: 83-86, 1950.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation