Plasmodium fieldi Eyles et al. (ATCC® 30157)

Strain Designations: A. hackeri  /  Depositor: National Institutes of Health  /  Biosafety Level: 2

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Strain Designations A. hackeri
Application
Vector borne research
Biosafety Level 2
Isolation
mosquito, West Malaysia
Product Format frozen
Type Strain no
Comments
Observation on the relapse activity
Growth Conditions
Duration: in vivo, Macaca mulatta
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Subcultivation
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Name of Depositor National Institutes of Health
References

Collins WE, Contacos PG. Observations on the relapse activity of Plasmodium fieldi in the rhesus monkey. J. Parasitol. 57: 29-32, 1971. PubMed: 4994804

Coatney GR. The primate malarias. Washington, DC: Government Printing Office; 1971.

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