Plasmodium simiovale Dissanaike et al. (ATCC® 30140)

Organism: Plasmodium simiovale Dissanaike et al.  /  Depositor: National Institutes of Health

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Application
Vector borne research
Biosafety Level 2
Isolation
Macaca sinica, Ceylon, 1965
Product Format frozen
Type Strain no
Comments
Studies on the exoerythrocytic stages
Growth Conditions
Duration: in vivo, Macaca mulatta
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Subcultivation
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Name of Depositor National Institutes of Health
Chain of Custody
ATCC <<--National Institutes of Health<<--Section on Primate Malaria, NIH A.S. Dissanai/P. Nelson/P.C.C. Garnham
Year of Origin 1965
References

Coatney GR. The primate malarias. Washington, DC: Government Printing Office; 1971.

Dissanaike AS, et al. Plasmodium simiovale sp. nov., a new simian malaria parasite from Ceylon. Ceylon J. Med. Sci. 14: 27-33, 1965.

Collins WE, et al. Studies on the exoerythrocytic stages of simian malaria. VII. Plasmodium simiovale. J. Parasitol. 58: 135-141, 1972. PubMed: 4622280

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