Plasmodium cynomolgi Mayer (ATCC® 30129)

Organism: Plasmodium cynomolgi Mayer  /  Depositor: National Institutes of Health

Strain Designations B
Application
Vector borne research
Biosafety Level 2
Isolation
Macaca fascicularis, West Malaysia, 1959
Product Format frozen
Type Strain no
Growth Conditions
Duration: in vivo, Macaca mulatta
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Subcultivation
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Name of Depositor National Institutes of Health
Chain of Custody
ATCC <<--National Institutes of Health<<--P.C.C. Garnham
Year of Origin 1959
References

Eyles DE. The species of simian malaria: taxonomy, morphology, life cycle, and geographical distribution of the monkey species. J. Parasitol. 49: 866-887, 1963. PubMed: 14084190

Collins WE, et al. Studies on the transmission of simian malaria. V. Infection and transmission of the B strain of Plasmodium cynomolgi. J. Parasitol. 58: 653-659, 1972. PubMed: 4626891

Riv. Parassitol. 20: 273-278, 1959.

Krotoski WA, et al. Immunofluorescent staining of exoerythrocytic schizonts of simian plasmodia in fixed tissue. Am. J. Trop. Med. Hyg. 22: 159-162, 1973. PubMed: 4120116

Rutledge LC, et al. Plasmodium cynomolgi: sources of variation in susceptibility of Anopheles quadrimaculatus, A. balabacensis, and A. stephensi. Exp. Parasitol. 27: 53-59, 1970. PubMed: 5413008