Plasmodium vinckei vinckei Rhodain (ATCC® 30091)

Depositor: M Yoeli  /  Biosafety Level: 1

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Application
Vector borne research
Biosafety Level 1
Isolation
mosquito, Anopheles dureni, Kinga River, Katanga, Zaire, 1952
Product Format frozen
Type Strain no
Comments
Not capable of developing in mosquito vectors.
cellular Mechanism in the Immune Response
Serial passage in white mice. Inject contents of ampule intraperitoneally into 2 or 3 white mice.
Growth Conditions
Duration: in vivo, laboratory mouse
Cryopreservation

CRYOPRESERVATION: 

Only young cells (rings) can be frozen in glycerolyte medium* because their membranes are more robust.

1.   To harvest parasites, inject host with ketamine (0.1–0.2 ml).

2.   Open chest cavity to expose heart and exsanguinate via cardiac puncture using Yaeger's anticoagulant** (see below), 1 volume anticoagulant to 4 volumes blood.                                                                                                                                                             Glass distilled HGla                                                                                                                                                                             

3.   Centrifuge blood for 5 mins. at 1800 rpm in 50 ml centrifuge tube.

4.   Aspirate supernatant using sterile Pasteur pipet.

5.   Resuspend pellet gently in remaining supernatant.

6.   Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows:

A.  Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.

B.  Add the remaining 4 volumes of glycerolyte and gently agitate.

7.   Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1°C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.).

8.   Plunge vials into liquid nitrogen (-196oC) the next day and store in liquid nitrogen or liquid nitrogen vapor.

       

Name of Depositor M Yoeli
Chain of Custody
ATCC <<--M Yoeli<<--I.H. Vincke
Year of Origin 1952
References

Nussenzweig RS, et al. Studies on the protective effect of Plasmodium chabaudi infection in mice upon a subsequent infection with another rodent malaria species, Plasmodium vinckei. Mil. Med. 131: 1237-1242, 1966. PubMed: 4957831

Yoeli M, Most H. A study of inapparent infections of Plasmodium vinckei in the adult hamster (Mesocricetus auratus). Trans. R. Soc. Trop. Med. Hyg. 64: 276-278, 1970. PubMed: 5449053

Wasserman GM, et al. An approach to development of specific t-lymphocyte lines by use of preprocessed antigens in Plasmodium vinckei vinckei murine malaria. Infect. Immun. 61: 1958-1963, 1993. PubMed: 8478085

Perlmann H, et al. Cellular mechanisms in the immune response to malaria in Plasmodium vinckei-infected mice. Infect. Immun. 63: 3987-3993, 1995. PubMed: 7558309

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation