Trypanosoma mega Dutton and Todd (ATCC® 30038)

Depositor: HN Guttman  /  Biosafety Level: 1

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Application
Vector borne research
Biosafety Level 1
Isolation
toad, Bufo regularis, Africa
Product Format frozen
Type Strain no
Comments
Phylogenetic relationships among anuran trypanosomes
Polyamine function
Cysteine and metalloproteinase activities
Genus identification based on specific 18SRibosomal RNA sequence
Polyamines
Karyotype analysis of anuran trypanosomes
enzymes of ornithine-arginine metabolism
Glycosphingolipid
Proteolytic activities
Acetylornithinase and ornithine acetyltransferase
Medium Medium 1011: Diphasic blood agar medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor HN Guttman
Chain of Custody
ATCC <<--HN Guttman<<--M. Steinert
References

Clark CG, et al. Phylogenetic relationship among anuran Trypanosoma as revealed by riboprinting. J. Eukaryot. Microbiol. 42: 92-96, 1995. PubMed: 7728144

Nature 178: 308-309, 1956.

Bacchi CJ, et al. Polyamine function in trypanosomatids: activation of cytoplasmic alpha-glycerophosphate dehydrogenase by cationic trypanocides. Adv. Polyamine Res. 2: 129-138, 1978.

Lopez TD. Volumen celular y adn de Tripanosomas. Acta Macarao 1(1): 1-12, 1973.

Lopez TD. Volumen celular y contenido de adn de Trypanosoma lewisi y T. mega in vitro. Acta Macarao 1(1): 13-20, 1973.

Camargo EP, et al. Proteolytic activities in cell extracts of trypanosomatids. J. Parasitol. 64: 1120-1121, 1978. PubMed: 739304

Branquinha MH, et al. Ubiquity of cysteine-and metalloproteinase activites in a wide range of trypanosomatids. J. Eukaryot. Microbiol. 43: 131-135, 1996. PubMed: 8720943

Uliana SR, et al. Leishmania: genus identification based on a specific sequence of the 18S ribosomal RNA sequence. Exp. Parasitol. 72: 157-163, 1991. PubMed: 2009920

Bacchi CJ, et al. Polyamines in trypanosomatids. J. Bacteriol. 131: 657-661, 1977. PubMed: 885842

Galinari S, Camargo EP. Trypanosomatid protozoa: survey of acetylornithinase and ornithine acetyltransferase. Exp. Parasitol. 46: 277-282, 1978. PubMed: 569594

Lun ZR, Desser SS. Karyotype analysis of anuran trypanosomes by pulsed-field gradient gel electrophoresis. J. Parasitol. 81: 1018-1020, 1995. PubMed: 8544044

Camargo EP, et al. Trypanosoma spp., Leishmania spp. and Leptomonas spp.: enzymes of ornithine-arginine metabolism. Exp. Parasitol. 46: 141-144, 1978. PubMed: 569593

Vermelho AB, et al. Isolation and characterization of a neutral glycosphingolipid from the epimastigote form of Trypanosoma mega. J. Protozool. 33: 208-213, 1986. PubMed: 3735150

Cross References

Nucleotide (GenBank) : AJ223567 Trypanosoma mega, small subunit ribosomal RNA gene.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation