Trypanosoma equiperdum Doflein (ATCC® 30019)

Organism: Trypanosoma equiperdum Doflein  /  Depositor: EJ Tobie

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Strain Designations NIH kinetoplastic
Application
Vector borne research
Biosafety Level 3
Product Format frozen
Type Strain no
Comments
Kinetoplast DNA in dyskinetoplastic strains
Abnormal mitochondrial RNA editing
Growth Conditions
Duration: in vivo, laboratory rat
Protocol: ATCCNO: 30018 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject 0.2 ml aliquots of the thawed contents of the ampule intraperitoneally into an adult rats known to be free of blood parasites. Draw a drop of blood from the tail of an infected rat. Add 1.0 ml of mammalian saline (0.8% saline buffered to pH 7.0) to the blood and examine a small amount microscopically. If one or more parasites are seen, proceed as follows: collect parasites into a syringe containing a buffered anticoagulant by either cardiac puncture or from the tail vein of the infected animal. Inoculate 0.2 ml of the diluted blood intraperitoneally into an adult rat known to be free of other blood parasites. Repeat the above procedures approximately every 4 days.
Subcultivation
Protocol: ATCCNO: 30018 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject 0.2 ml aliquots of the thawed contents of the ampule intraperitoneally into an adult rats known to be free of blood parasites. Draw a drop of blood from the tail of an infected rat. Add 1.0 ml of mammalian saline (0.8% saline buffered to pH 7.0) to the blood and examine a small amount microscopically. If one or more parasites are seen, proceed as follows: collect parasites into a syringe containing a buffered anticoagulant by either cardiac puncture or from the tail vein of the infected animal. Inoculate 0.2 ml of the diluted blood intraperitoneally into an adult rat known to be free of other blood parasites. Repeat the above procedures approximately every 4 days.
Name of Depositor EJ Tobie
Chain of Custody
ATCC <<--EJ Tobie<<--USDA <<--- Pasteur Institut 1903 (?)
References

Hajduk SL. Demonstration of kinetoplast DNA in dyskinetoplastic strains of Trypanosoma equiperdum. Science 191: 858-859, 1976. PubMed: 1251198

Tobie EJ. Loss of the kinetoplast in a strain of Trypanosoma equiperdum. Trans. Am. Microsc. Soc. 70: 251-254, 1951.

Shu HH, Stuart K. Mitochondrial transcripts are processed but are not edited normally in Trypanosoma equiperdum (ATCC 30019) which has kDNA sequence deletion and duplication. Nucleic Acids Res. 22: 1696-1700, 1994. PubMed: 8202373

Cross References

Nucleotide (GenBank) : U03740 Trypanosoma equiperdum kinetoplast maxicircle CR4 region.

Nucleotide (GenBank) : U03739 Trypanosoma equiperdum kinetoplast maxicircle variable region.

Nucleotide (GenBank) : U03738 Trypanosoma equiperdum kinetoplast maxicircle rearranged ND4-ND5 region.

Nucleotide (GenBank) : U03741 Trypanosoma equiperdum kinetoplast maxicircle 12S rRNA gene/COI gene deletion junction.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
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