Canine adenovirus 2 (ATCC® VR-800)

Classification: Adenoviridae, Mastadenovirus, Canine adenovirus A  /  Product Format: frozen 1 mL per vial

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Classification Adenoviridae, Mastadenovirus, Canine adenovirus A
Deposited As Canine adenovirus
Agent Canine adenovirus 2
Strain Toronto A 26/61
Applications
Respiratory research
Biosafety Level 2
Isolation
Throat swab from a dog with laryngotracheitis
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-800 Images
Effect on Host CPE, cell rounding and sloughing
Recommended Host MDCK (ATCC CCL-34)
Growth Conditions Temperature: 37°C

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum.  End adsorption by adding virus growth medium.

Incubation: 3-8 days
Effect on Host CPE, cell rounding and sloughing
Name of Depositor LN Binn, Walter Reed Army Institute of Research, Washington, DC
Source Throat swab from a dog with laryngotracheitis
References

Ditchfield J, et al. Association of a canine adenovirus (Toronto A 26/61) with an outbreak of laryngotracheitis (“Kennel Cough”): A Preliminary Report. Can. Vet. J. 3: 238-247.1962, PubMed: 17421510

Kapsenberg JG. Relationship of infectious canine hepatitis virus to human adenovirus. Proc. Soc. Exp. Biol. Med. 101: 611-614, 1959. PubMed: 14404448

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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