Bovine adenovirus 4 (ATCC® VR-640)

Classification: Adenoviridae, Atadenovirus,  / 

Classification Adenoviridae, Atadenovirus,
Deposited As Bovine adenovirus type 4
Agent Bovine adenovirus 4
Strain THT/62
Applications
Respiratory research
Biosafety Level 2
Isolation
Lungs of a calf with pneumoenteritis
Storage Conditions  -70°C or colder
Comments
Does not share common CF antigen with human adenoviruses. No cross-reactivity with bovine adenovirus types 1-3.
GenBank: AF036092.3
Effect on Host
CPE, generalized enlarged cell rounding and cell clumping or clustering; Type B intranuclear inclusion bodies
 CPE, generalized enlarged cell rounding and cell clumping or clustering; Type B intranuclear inclusion bodies
Recommended Host
BT (ATCC CRL-1390)

Production Host:  BT (ATCC® CRL-1390)

Growth Conditions
Temperature: 37.0°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C without CO2, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 6-7 days at 37°C without CO2, until 90% of the culture shows CPE.
Effect on Host
CPE, generalized enlarged cell rounding and cell clumping or clustering; Type B intranuclear inclusion bodies
 CPE, generalized enlarged cell rounding and cell clumping or clustering; Type B intranuclear inclusion bodies
Name of Depositor SB Mohanty
Chain of Custody
Deposited as Bovine adenovirus type 4. Originally received after 6 passages in primary calf testicular cells.
Source
Lungs of a calf with pneumoenteritis
References

Bartha A, Aldasy P. Further two serotypes of bovine adenoviruses (serotype 4 and 5). Acta Vet. Acad. Sci. Hung. 16(1): 107-108, 1966. PubMed: 5917816

Cross References

GenBank : AF036092.3 Bovine adenovirus 4 strain THT/62, complete genome.