Bovine adenovirus 4 (ATCC® VR-640)

Classification: Adenoviridae, Atadenovirus,  / 

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Classification Adenoviridae, Atadenovirus,
Deposited As Bovine adenovirus type 4
Agent Bovine adenovirus 4
Strain THT/62
Applications
Respiratory research
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Lungs of a calf with pneumoenteritis
Storage Conditions  -70°C or colder
Comments
Does not share common CF antigen with human adenoviruses. No cross-reactivity with bovine adenovirus types 1-3.
GenBank: AF036092.3
Effect on Host
CPE, generalized enlarged cell rounding and cell clumping or clustering; Type B intranuclear inclusion bodies
 CPE, generalized enlarged cell rounding and cell clumping or clustering; Type B intranuclear inclusion bodies
Recommended Host
BT (ATCC CRL-1390)

Production Host:  BT (ATCC® CRL-1390)

Growth Conditions
Temperature: 37.0°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C without CO2, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 6-7 days at 37°C without CO2, until 90% of the culture shows CPE.
Effect on Host
CPE, generalized enlarged cell rounding and cell clumping or clustering; Type B intranuclear inclusion bodies
 CPE, generalized enlarged cell rounding and cell clumping or clustering; Type B intranuclear inclusion bodies
Name of Depositor SB Mohanty
Chain of Custody
Deposited as Bovine adenovirus type 4. Originally received after 6 passages in primary calf testicular cells.
Source
Lungs of a calf with pneumoenteritis
References

Bartha A, Aldasy P. Further two serotypes of bovine adenoviruses (serotype 4 and 5). Acta Vet. Acad. Sci. Hung. 16(1): 107-108, 1966. PubMed: 5917816

Cross References

GenBank : AF036092.3 Bovine adenovirus 4 strain THT/62, complete genome.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation