Bovine adenovirus 1 (ATCC® VR-313)

Classification: Adenoviridae, Mastadenovirus, Bovine mastadenovirus A  /  Product Format: frozen 1.0 mL

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Classification Adenoviridae, Mastadenovirus, Bovine mastadenovirus A
Deposited As Bovine adenovirus type 1
Agent Bovine adenovirus 1
Strain 10
Applications
Respiratory research
Biosafety Level 2
Isolation
Feces of normal calf
Product Format frozen 1.0 mL
Storage Conditions -70°C or colder
Images ATCC VR-313 images
Comments REFERENCE STRAIN: Pereira HG et al. A short description of the Adenovirus group. Virology 20: 613-620, 1963.

Agglutinates rat RBC. CPE may be minimal, frequently demonstrating less than 25% involvement of cell sheet, even with prolonged incubation.  AAV reported to be present.
Effect on Host
CPE, cell rounding with vacuolization, clumping, and sloughing
Recommended Host
MDBK (NBL-1) cells (ATCC® CCL-22™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 4-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, cell rounding with vacuolization, clumping, and sloughing
Name of Depositor M Klein
Source Feces of normal calf
References

Klein M, et al. Isolation from cattle of a virus related to human adenovirus. Proc. Soc. Exp. Biol. Med. 102: 1-4, 1959. PubMed: 14409689

Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
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