Canine distemper virus (ATCC® VR-1587)

Classification: Paramyxoviridae, Morbillivirus  /  Product Format: frozen

Classification Paramyxoviridae, Morbillivirus
Agent Canine distemper virus
Strain Snyder Hill (cell culture adapted)
Applications
It is used primarily as a challenge strain to test dogs for immunity to the disease.
VR-526, deposited by L. E. Carmichael, was originally isolated from the brain of a dog with clinical distemper in September, 1950. This virus produces neurological signs with regularity only when inoculated intracerebrally.
It does not produce hemagglutinin or hemolysin.
Respiratory research
Positive for Mycoplasma No
Biosafety Level 2
Isolation
VR-1587 was derived at the ATCC from VR-526 by adaptation to growth in Vero cells (ATCC CCL-81). VR-526, deposited by L. E. Carmichael, was originally isolated from the brain of a dog with clinical distemper in September, 1950. This virus produces neurological signs with regularity only when inoculated intracerebrally. It does not produce hemagglutinin or hemolysin. It is used primarily as a challenge strain to test dogs for immunity to the disease. Cross-immunity tests in dogs have shown this strain to be similar to other classical strains of CDV.
Product Format frozen
Storage Conditions -70°C or colder
Comments
VR-1587 was derived at the ATCC from VR-526 by adaptation to growth in Vero cells (ATCC CCL-81). VR-526, deposited by L. E. Carmichael, was originally isolated from the brain of a dog with clinical distemper in September, 1950. This virus produces neurological signs with regularity only when inoculated intracerebrally. It does not produce hemagglutinin or hemolysin. It is used primarily as a challenge strain to test dogs for immunity to the disease. Cross-immunity tests in dogs have shown this strain to be similar to other classical strains of CDV.
Effect on Host
Yes, Cytopathic effect: vacuolization, rounding and sloughing of host cells
CPE, vacuolization and rounding of cells, sloughing
Recommended Host

Recommended Host: Vero cells (ATCC® CCL-81™)

Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Duration: 5-7 days
Cryopreservation
Storage temperature: -70 or colder
Effect on Host
Yes, Cytopathic effect: vacuolization, rounding and sloughing of host cells
CPE, vacuolization and rounding of cells, sloughing
Name of Depositor LE Carmichael, ATCC
Special Collection ATCC
References

Gillespie JH. . Am. J. Vet. Res. 17: 103, 1956. PubMed: 13259024