Canine parainfluenza virus (ATCC® VR-1573)

Classification: Paramyxoviridae, Paramyxovirinae, Unclassified  /  Product Format: frozen

Classification Paramyxoviridae, Paramyxovirinae, Unclassified
Agent Canine parainfluenza virus
Strain 78-238
Applications
Respiratory research
Biosafety Level 2
Isolation
derived from existing strain
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Comments
This an encephalitogenic strain of Canine parainfluenza virus.
Effect on Host
Yes, in vitro effects: cytopathic effect, syncytial sloughing
CPE, syncytial sloughing
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host

Recommended Host:  ATCC® CCL-81™ (Vero)

Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 36.0°C
Duration: 3-4 days; For best results cells should be 24 to 48 hours old and 70-80% confluent (not 100% confluent).
Cryopreservation
Storage temperature: -70°C
Effect on Host
Yes, in vitro effects: cytopathic effect, syncytial sloughing
CPE, syncytial sloughing
Name of Depositor ATCC, JF Evermann
Special Collection ATCC
Source
derived from existing strain
References

Evermann JF, et al. Isolation of a paramyxovirus from the cerebrospinal fluid of a dog with posterior paresis. J. Am. Vet. Med. Assoc. 177: 1132-1134, 1980. PubMed: 6254938

Evermann JF, et al. Properties of an encephalitogenic canine parainfluenza virus. Arch. Virol. 68: 165-172, 1981. PubMed: 6168243

or colder

ATCC VR-963 was filtered to remove bacterial and fungal contaminants and reaccessioned as ATCC VR-1573. ATCC VR-963 was isolated from cerebrospinal fluid from dog with posterior paresis.

or colder