Canine distemper virus (ATCC® VR-128)

Classification: Paramyxoviridae, Morbillivirus  /  Product Format: frozen 1 mL per vial

Classification Paramyxoviridae, Morbillivirus
Deposited As Canine distemper
Agent Canine distemper virus
Strain Lederle
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images ATCC VR-128 Canine distemper virus
Comments
Belongs to the same virus subgroup as measles and rinderpest.
Effect on Host
CPE, refractile cell rounding and degeneration
Recommended Host
SL-29 (ATCC® CRL-1590™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 5-8 days. Replace virus growth medium every 2-3 days.
Effect on Host
CPE, refractile cell rounding and degeneration
Name of Depositor VJ Cabasso, PJ Vasington, Lederle Laboratories Division of American Cyanamid Company
Source Field case of distemper encephalitis in dog
References

Cabasso VJ, Cox HR. A distemper-like strain of virus derived from a case of canine encephalitis: its adaptation to the chick embryo and subsequent modification. Cornell Vet. 42: 96-107, 1952. PubMed: 14905890