WIL2-S (ATCC® CRL-8885™) Organism: Homo sapiens, human / Tissue: spleen / Cell Type: B lymphoblast General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism Homo sapiens, human Tissue spleen Cell Type B lymphoblast Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 2 [Cells contain Epstein-Barr virus] Disease hereditary spherocytosis Age 5 years Gender male Ethnicity Caucasian Applications This cell line is a suitable transfection host. Storage Conditions liquid niotrogen vapor phase Clinical Data 5 yearsCaucasianmale Comments This is a HAT sensitive variant of the WIL2 human B cell line (see also ATCC CRL-8155). Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/mL. Medium Renewal: Every 2 to 3 days STR Profile Amelogenin: X,YCSF1PO: 11,12D13S317: 11D16S539: 11,12D5S818: 12,13D7S820: 9,11F13A01: 6,7F13B: 10FESFPS: 11,12LPL: 9,10THO1: 8,9.3TPOX: 8,11vWA: 17,20 Name of Depositor Lovelace Medical Foundation Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS